Human Protocadherin gamma C3 Antibody

Catalog #: MAB83642 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB83642-100
MAB83642-SP

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Human Protocadherin gamma C3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protocadherin gamma C3 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 926531

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Protocadherin gamma C3 protein
Ser30-Tyr693
Accession # Q9UN70

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (medulla)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Neurogranin in Human Brain (Medulla). Neurogranin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Neurogranin Monoclonal Antibody (Catalog # MAB79471) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin gamma C3

Protocadherin gamma C3 is a member of the gamma subgroup of clustered protocadherins (1). Like other gamma protocadherins, mature Protocadherin gamma C3 contains six extracellular cadherin domains, a transmembrane region, and a cytoplasmic domain (2, 3). Within the ECD, human Protocadherin gamma C3 shares 91% and 92% amino acid sequence identity with mouse and rat Protocadherin gamma C3, respectively. It plays an important role in cell adhesion and cell recognition through CA²⁺ -dependent homophilic interaction (4). MMP-mediated shedding of gamma protocadherins and release of their cytoplasmic domain by the gamma -secretase complex results in translocation of the intracellular domain into the nucleus and transcriptional activation of target genes (5-7). Protocadherin gamma C3 is cleaved within its ectodomain by ADAM10 in fibroblasts and neuronal cells (8). Deletion of the entire protocadherin gamma gene cluster is embryonic lethal in mice (9). Protocadherin gamma C3 is most notably expressed in the nervous system (10). Conditional deletion of the protocadherin gamma gene cluster in mice affects development of retinal ganglion cells and spinal cord interneurons, resulting in decreased synapses and increased neuronal apoptosis (9, 11-14). The C-type protocadherin gamma isoforms specifically may be responsible for the increased apoptosis observed in mice lacking the entire protocadherin gamma gene cluster (15). Cortical neuron-specific deletion of the protocadherin gamma gene cluster results in dendritic arborization defects (16). The protocadherin gamma subfamily may also be involved in cerebrospinal fluid production and the maturation and differentiation of postnatally born olfactory granule cells (17, 18).

References

Yagi, T. (2008) Dev. Growth Differ. 50:S131.
Sano, K. et al. (1993) EMBO J. 12:2249.
Kohmura, N. et al. (1998) Neuron 20:1137.
Obata, S. et al. (1995) J. Cell Sci. 108:3765.
Hambsch, B. et al. (2005) J. Biol. Chem. 280:15888.
Haas, I.G. et al. (2005) J. Biol. Chem. 280:9313.
Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.
Reiss, K. et al. (2006) J. Biol. Chem. 281:21735.
Wang, X. et al. (2002) Neuron 36:843.
Phillips, G.R. et al. (2003) J. Neurosci. 23:5096.
Weiner, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:8.
Lefebvre, J.L. et al. (2008) Development 135:4141.
Prasad, T. et al. (2008) Development 135:4153.
Lin, C. et al. (2010) J. Biol. Chem. 285:41675.
Chen, W.V. et al. (2012) Neuron 75:402.
Garrett, A.M. et al. (2012) Neuron 74:269.
Lobas, M.A. et al. (2012) J. Neurochem. 120:913.
Ledderose, J. et al. (2013) Sci. Rep. 3:1514.

Entrez Gene IDs

5098 (Human); 93706 (Mouse); 116782 (Rat)

Alternate Names

Cadherin-Like 2; PC43; PC-43; PCDH2; PCDH-GAMMA-C3; PCDHGC3; protocadherin 2; Protocadherin 43; Protocadherin gamma C3; protocadherin gamma subfamily C, 3; protocadherin gamma-C3; Protocadherin-2; protocadherin-43