Human PTGDR Antibody

Catalog #: MAB11249 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11249-100
MAB11249-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTGDR Antibody Summary

Specificity

Detects Prostaglandin D2 receptor immunogen in Direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1052528

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide within amino acids 50-150 of the Prostaglandin D2 receptor protein sequences
Accession # Q13258

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Colon.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of PTGDR in Human Colon. PTGDR was detected in immersion fixed paraffin-embedded sections of Human Colon using Mouse Anti-Human PTGDR Monoclonal Antibody (Catalog # MAB11249) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGDR

PTGDR is a G protein-coupled receptor that has been shown to function as a prostanoid DP receptor. PTGDR is expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes. The activity of this receptor is mainly mediated by G-S proteins that stimulate adenylate cyclase resulting in an elevation of intracellular cAMP and Ca2+. Knockout studies in mice suggest that the ligand of PTGDR, prostaglandin D2 (PGD2), functions as a mast cell-derived mediator to trigger asthmatic responses. Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea.

Long Name

Prostaglandin D2 Receptor

Entrez Gene IDs

5729 (Human); 19214 (Mouse); 498475 (Rat)

Alternate Names

AS1; ASRT1; DP; DP1; MGC49004; PGD receptor; PGD2 receptor; prostaglandin D2 receptor (DP); prostaglandin D2 receptor; Prostanoid DP receptor; PTGDR; PTGDR1