Human PTGER2 Antibody

Catalog #: MAB6656 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6656
MAB6656-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTGER2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTGER2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 607077

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human PTGER2
Accession # P43116

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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PTGER2 in Human Bladder. PTGER2 was detected in immersion fixed paraffin-embedded sections of human bladder using Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Strongest staining was localized to umbrella cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot

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Detection of Human PTGER2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTGER2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGER2

Prostaglandin E2 receptor (PTGER2) is a 53 kDa member of the G-protein coupled receptor family of proteins. Human PTGER2 is 358 amino acids (aa) in length and has the seven putative transmembrane domains characteristic of G-protein coupled receptors. In addition, there are four potential sites for N-linked glycosylation. Human PTGER2 shares 86% and 83% aa sequence identity with mouse and rat PTGER2, respectively. PTGER2 is present in the vasculature, the gastrointestinal, tracheobronchial, and reproductive tracts, the kidney, and also in the sphincter, dilator, and ciliary muscles in the eye. PTGER2 is linked to prostaglandin E2-mediated vasodilation and smooth muscle relaxation. It is also involved in the stimulation of secretion in the stomach and small intestine, the control of intraocular pressure, and the regulation of bone resorption.

Long Name

Prostaglandin E2 Receptor 2, Subtype EP2

Entrez Gene IDs

5732 (Human); 19217 (Mouse); 81752 (Rat)

Alternate Names

EP2; PGE receptor EP2 subtype; PGE2 receptor EP2 subtype; prostaglandin E receptor 2 (subtype EP2), 53kD; prostaglandin E receptor 2 (subtype EP2), 53kDa; prostaglandin E2 receptor EP2 subtype; Prostanoid EP2 receptor; PTGER2