Human PUM2 Antibody

Catalog #: AF7415 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7415
AF7415-SP

Detection of Human PUM2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PUM2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PUM2 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PUM1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PUM2
Pro103-Gly250
Accession # Q8TB72

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PUM2 by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PUM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PUM2 at approximately 115-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PUM2

PUM2 (Pumilio homolog 2/Pumilio-2) is a 121-125 kDa member of the PUF family of RNA-binding peoteins. It is expressed in a variety of cell types, including neurons, skeletal muscle cells, stem cells and germ cells, and plays a role in asymmetric cell division. PUM2 appears to bind to the 3' untranslated region of target mRNAs. By doing so, it causes a conformational change in the RNA, allowing for the subsequent binding of miRNAs and the silencing of protein translation. In germ cells, PUM2 is also reported to complex with NANOSI and SNAPIN, although its function in this complex is unclear. Human PUM2 is 1066 amino acids (aa) in length. It contains a SNAPIN interaction site (aa 1-260), one PUM-HD region (aa 706-1048) and eight consecutive pumilio repeats (aa 726-1022). There are six utilized Ser/Thr phosphorylation sites, and at least two isoform variants. One is 95-96 kDa in size and shows a deletion of aa 574-652, while another shows a deletion of two amino acids; Val829Ile830. Over aa 103-250, human PUM2 shares 95% aa sequence identity with mouse PUM2.

Long Name

Pumilio 2

Entrez Gene IDs

23369 (Human); 80913 (Mouse); 298874 (Rat)

Alternate Names

FLJ36528; KIAA0235PUMH2PUML2; MGC138251; MGC138253; PUM2; PUMH2; pumilio (Drosphila) homolog 2; pumilio homolog 2 (Drosophila); pumilio homolog 2; Pumilio-2; PUML2