Human PVRIG Antibody

Catalog #: MAB93651 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB93651-100
MAB93651-SP

Detection of PVRIG in HEK293 Human Cell Line Transfected with Human PVRIG and eGFP by Flow Cytometry.

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All PVRIG Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PVRIG Antibody Summary

Species Reactivity

Human

Specificity

Detects human PVRIG in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2334A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human PVRIG protein
Thr 41 & Glu43-Leu172
Accession # Q6DK17

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of PVRIG in HEK293 Human Cell Line Transfected with Human PVRIG and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human PVRIG or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human PVRIG Monoclonal Antibody (Catalog # MAB93651) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PVRIG

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

References

Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Alternate Names

C7orf15; C7orf15MGC138295; CD112R; MGC104322; MGC138297; MGC2463; poliovirus receptor related immunoglobulin domain containing; Poliovirus receptor-related immunoglobulin domain-containing protein; PVRIG; transmembrane protein PVRIG