Human R-Spondin 2 Antibody

Catalog #: AF3266
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3266
AF3266-SP

All R-Spondin 2 Antibodies

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human R-Spondin 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human R-Spondin 2 in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant human (rh) R-Spondin 1, rhR-Spondin 3, and recombinant mouse R-Spondin 4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human R-Spondin 2 isoform 1
Cys21-Gly205
Accession # Q6UXX9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human R-Spondin 2 (Catalog # 3266-RS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 2

Human Roof plate-specific Spondin 2 isoform 1 (R-Spondin 2, RSPO-2), also known as cysteine-rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family. The four known human R-Spondins regulate beta -catenin signaling and overlap in expression and function (1‑3). Like other R-Spondins, R-Spondin 2 contains two adjacent cysteine-rich furin-like domains (aa 90-134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207-243). The basic region appears to mediate cell surface retention but not to influence function (1). R‑Spondin 2 contains one potential N-glycosylation site. Of the three reported splice isoforms of human R-Spondin 2, isoform 2 lacks residues 1-67 of isoform 1, while isoform 3 has a glycine substitution for residues 32-95 of isoform 1. Human R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is downregulated in tumors of these tissues (1). In the embryonic mouse, R-Spondin 2 is expressed most highly in the hippocampus and in developing teeth and bones (4). Studies in Xenopus and mouse embryos indicate that R-Spondin 2 is an extracellular activator of Wnt/ beta -catenin signaling and is required for myogenesis (1). Mouse R-Spondin proteins bind both LRP-6 and Frizzled-8 but do not appear to form a ternary complex (3). R-Spondin 2 over-expression in Xenopus also blocks signaling of TGF-beta ligands, activin, nodal and BMP-4 (1). Human R-Spondin 2 is highly conserved across species, sharing 97-98% aa identity with mouse, rat, canine, bovine, and opossum R-Spondin 2 and 86% aa identity with Xenopus R-Spondin 2 within aa 22-205. Mature R-Spondin 2 shares ~40% aa identity with R-Spondin 1, R-Spondin 3, and R-Spondin 4.

References

Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
Kim, K-A. et al. (2006) Cell Cycle 5:23.
Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
Nam, J-S. et al. (2007) Gene Expr. 281:13247.

Long Name

Roof Plate-specific Spondin 2

Entrez Gene IDs

340419 (Human); 239405 (Mouse)

Alternate Names

Cristin 2; CRISTIN2; hRspo2; MGC35555; MGC43342; Roof plate-specific spondin-2; RSPO2; R-spondin 2 homolog (Xenopus laevis); RSpondin 2; R-Spondin 2; R-spondin-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human R-Spondin 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/?-catenin pathway
Authors: Guo, D;Pan, H;Lu, X;Chen, Z;Zhou, L;Chen, S;Huang, J;Liang, X;Xiao, Z;Zeng, H;Shao, Y;Qi, W;Xie, D;Lin, C;
Arthritis research & therapy
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
RSPO2 inhibits BMP signaling to promote self-renewal in acute myeloid leukemia
Authors: R Sun, L He, H Lee, A Glinka, C Andresen, D Hübschmann, I Jeremias, K Müller-Dec, C Pabst, C Niehrs
Cell Reports, 2021-08-17;36(7):109559.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring.
Authors: Chua AW, Ma D, Gan SU, Fu Z, Han HC, Song C, Sabapathy K, Phan TT
J. Invest. Dermatol., 2010-12-16;131(3):644-54.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot