Human Rad50 Antibody

Catalog #: MAB4996 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4996
MAB4996-SP

Detection of Human Rad50 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Rad50 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Rad50 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Rad51 or rhRad53 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 684945

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rad50
Leu518-Leu881
Accession # Q92878

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

HeLa human cervical epithelial carcinoma cells and HepG2 human hepatocellular carcinoma cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Rad50 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cells, HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Rad50 Monoclonal Antibody (Catalog # MAB4996) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rad50 at approximately ~150kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad50

Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes. It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex. This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells. Human Rad50 is 1312 amino acids (aa) in length. It has an apparent ATP binding site (aa 36-43) plus a coiled-coil region (aa 228-598) followed by a “zinc-hook” domain (aa 635-734) that mediates homodimerization. There are multiple splice variants. An alternate start site exists at Met140, there is a single Lys substitution for aa 723-1312, and three Lys substitute for aa 555-1312. Over aa 518-881, human Rad50 is 96% aa identical to mouse Rad50.

Entrez Gene IDs

10111 (Human); 19360 (Mouse); 64012 (Rat)

Alternate Names

DNA repair protein RAD50; EC 3.6; EC 3.6.1.15; EC 3.6.3; EC 3.6.3.27; hRad50; NBSLD; RAD50 (S. cerevisiae) homolog; RAD50 homolog (S. cerevisiae); Rad50; RAD502; RAD50-2