Human Rad51 Antibody

Catalog #: MAB9948 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9948-100
MAB9948-SP

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Human Rad51 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Rad51 in direct ELISAs.

Source

Monoclonal Mouse IgG₃ Clone # 523701

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rad51
Val174-Asp339
Accession # Q06609

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Rad51 in Human Breast Cancer Tissue. Rad51 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Rad51 Monoclonal Antibody (Catalog # MAB9948) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad51

Human Rad51 is a 339 amino acids, 37kDa protein that plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. RAD51 family members are homologous to the bacterial RecA and yeast Rad51 and are highly conserved in most eukaryotes, from yeast to humans. Different splice variants of the human RAD51 gene have been found.

Entrez Gene IDs

5888 (Human); 19361 (Mouse)

Alternate Names

BRCC5; HRAD51; HsRad51; HsT16930; RAD51 (S. cerevisiae) homolog (E coli RecA homolog); RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog A; Rad51; RAD51ABRCA1/BRCA2-containing complex, subunit 5; RAD51L3; RecA, E. coli, homolog of; RECADNA repair protein RAD51 homolog 1; RecA-like protein; recombination protein A