Human/Rat CCR4 PerCP-conjugated Antibody

Catalog #: FAB1567C
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1567C-025
FAB1567C-100

Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry.

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Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat CCR4 PerCP-conjugated Antibody Summary

Species Reactivity

Human, Rat

Specificity

Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.

Source

Monoclonal Mouse IgG_2B Clone # 205410

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CCR4 transfectants
Met1-Leu360
Accession # P51679

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Mouse Anti-Human/Rat CCR4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1567C) or (B) Mouse IgG_2BPerCP Isotype Control (Catalog # IC0041C). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR4 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Mouse Anti-Human/Rat CCR4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1567C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR4

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Entrez Gene IDs

1233 (Human); 12773 (Mouse); 171054 (Rat); 403541 (Canine); 102120178 (Cynomolgus Monkey)

Alternate Names

C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Rat CCR4 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes
Authors: T Nargis, K Kumar, AR Ghosh, A Sharma, D Rudra, D Sen, S Chakrabart, S Mukhopadhy, D Ganguly, P Chakrabart
Mol Metab, 2017-09-27;6(11):1529-1539.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry