Human/Rat DYRK1A Antibody

Catalog #: AF5407
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5407
AF5407-SP

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat DYRK1A Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat DYRK1A in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DYRK1A
Arg438-Thr511
Accession # Q13627

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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DYRK1A in Human Kidney. DYRK1A was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in rat samples.

Western Blot

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Detection of Human/Rat DYRK1A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DYRK1A at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human DYRK1A by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DYRK1A at approximately 113 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DYRK1A

DYRK1A (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 1A; also hMNB) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK1A is ubiquitously expressed and shows dual substrate specificity. Autophosphorylation on Tyr321 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include APP, Tau, STAT3, and Cyclin L2. Human DYRK1A is 763 amino acids (aa) in length. It contains two NLSs (aa 69‑105 and 109-150), a kinase catalytic domain (aa 159-479), and C-terminal poly-Ser and poly-His regions. DYRK1A activity has been implicated in neuronal development and Down syndrome.

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 1A

Entrez Gene IDs

1859 (Human); 13548 (Mouse); 25255 (Rat)

Alternate Names

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; DYRK1; DYRK1A; MNBEC 2.7.110EC 2.7.12; MNBH

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Rat DYRK1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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APP and DYRK1A regulate axonal and synaptic vesicle protein networks and mediate Alzheimer's pathology in trisomy 21 neurons
Authors: CI Wu, EA Vinton, RV Pearse, K Heo, AJ Aylward, YC Hsieh, Y Bi, S Adeleye, S Fancher, DM Duong, NT Seyfried, TL Schwarz, TL Young-Pear
Molecular Psychiatry, 2022-02-23;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Truncation and Activation of Dual Specificity Tyrosine Phosphorylation-regulated Kinase 1A by Calpain I: A MOLECULAR MECHANISM LINKED TO TAU PATHOLOGY IN ALZHEIMER DISEASE.
Authors: Jin N, Yin X, Gu J, Zhang X, Shi J, Qian W, Ji Y, Cao M, Gu X, Ding F, Iqbal K, Gong C, Liu F
J Biol Chem, 2015-04-27;290(24):15219-37.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot