Human/Rat GR/NR3C1 Antibody

Catalog #: MAB10144 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10144-100
MAB10144-SP

Detection of Human and Rat GR/NR3C1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat GR/NR3C1 Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human GR/NR3C1 in direct ELISAs. Detects human and rat GR/NR3C1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 1006903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GR/NR3C1
Val271-Lys777
Accession # P04150

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat GR/NR3C1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U-87 MG human glioblastoma/astrocytoma cell line, A549 human lung carcinoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GR/NR3C1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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GR/NR3C1 in HeLa Human Cell Line. GR/NR3C1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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GR/NR3C1 in Human Liver. GR/NR3C1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human GR/NR3C1 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GR/NR3C1 at approximately 97 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GR/NR3C1

GR is a glucocorticoid receptor that is expressed in almost every cell in the body and regulates genes controlling development, metabolism and the immune response. The unbound receptor resides in the cytosol of the cell, but upon binding to glucocorticoids, it is translocated to the nucleus where it regulates gene transcription. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or acts to repress the expression of pro-inflammatory proteins in the cytosol. It is also involved in chromatin remodeling and plays a role in rapid mRNA degradation.

Long Name

Glucocorticoid Receptor

Entrez Gene IDs

2908 (Human)

Alternate Names

GCR; glucocorticoid receptor; GR; GRGCCR; GRLNuclear receptor subfamily 3 group C member 1; NR3C1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); nuclear receptor subfamily 3, group C, member 1