Human/Rat Myoglobin Antibody

Catalog #: MAB9720 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9720-100
MAB9720-SP

Detection of Human and Rat Myoglobin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Myoglobin Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human Myoglobin in direct ELISAs. Detects human and rat Myoglobin in Western blots. In Western blots, no detection of mouse myglobin was observed.

Source

Monoclonal Mouse IgG_2A Clone # 974102

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Purified human cardiac Myoglobin antigen from human heart
Accession # P02144

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.05 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat Myoglobin by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue (negative control), rat heart tissue, and human liver tissue (negative control). PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Myoglobin at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Myoglobin in Human Heart. Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human and Rat Myoglobin by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue, rat heart tissue, and mouse heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Myoglobin at approximately 22 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat Myoglobin Monoclonal Antibody (Catalog # MAB9720). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myoglobin

Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.

Entrez Gene IDs

4151 (Human); 17189 (Mouse); 59108 (Rat)

Alternate Names

MB; MGC13548; Myoglobin; PVALB