Human/Rat Neuregulin-2/NRG2 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Neuregulin-2/NRG2

NRG2 (Neuregulin 2; also DON-1 and NTAK) is a member of a four gene family of ErbB binding proteins. It is expressed by spinal cord motor neurons, Schwann cells, cerebellar Purkinje cells, plus cerebellar and dentate granule cells. Notably on neurons, NRG2 is dendrite-associated. There are multiple isoforms for NRG2, and their expression appear to be cell-specific. NRG2 has a number of activities. It is reported to induce acetylcholine receptor transcription, and to either promote or inhibit cell proliferation in an isoform and cell-specific manner. Human NRG2 is synthesized as an 850 amino acid (aa) proprecursor, possibly representing a type III transmembrane (TM) protein. It possesses a 111 aa N-terminal prosequence, a 294 aa extracellular region (aa 112-405), and a 424 aa cytoplasmic domain (aa 427‑850). The extracellular region contains one Ig-like C2-type domain (aa 237-332) and an EGF-like domain (aa 341-382). As noted, NRG2 has at least 10 isoform variants, almost all of which are created by either the use of an alternative start site or exon splicing of the EGF domain. There are four main types; alpha  beta  gamma and δ. The isoform associated with this antibody is the  alpha form based on the peptide sequence covering aa 373-397. The  beta isoform, by contrast, possesses an 18-24 aa substitution over this sequence, while the  gamma (or potentially soluble) isoform contains a Gly substitution for aa 372-850, and the δ isoform shows a Glu substitution for the aa 331-397 of the Ig-like domain. It is suggested that TM NRG2 is proteolytically cleaved after Lys404, generating a 53 kDa cytosolic fragment and a 40-45 kDa soluble component that binds to cell-surface proteoglycans. Over aa 112-405, human NRG2( alpha ) shares 97% aa sequence identity with mouse NRG2( alpha ).

Product Datasheets

Related Research Areas

• EGF Family
• EGF Ligands