Human/Rat Neurogenin-2 Antibody

Catalog #: MAB3314
14 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3314
MAB3314-SP

Detection of Neurogenin‑2 in Human Cerebellum.

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All Neurogenin-2 Antibodies

Product Details

Citations (14)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Neurogenin-2 Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detect human and rat Neurogenin‑2.

Source

Monoclonal Mouse IgG_2A Clone # 7G4

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat Neurogenin-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human cerebellum and rat brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Neurogenin‑2 in Human Cerebellum. Neurogenin‑2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Mouse Anti-Human/Rat Neurogenin‑2 Monoclonal Antibody (Catalog # MAB3314) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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Detection of Neurogenin‑2 in Rat Brain. Neurogenin‑2 was detected in immersion fixed paraffin-embedded sections of rat brain using Mouse Anti-Human/Rat Neurogenin‑2 Monoclonal Antibody (Catalog # MAB3314) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurogenin-2

Neurogenin-2 (NgN2) is a member of neuroD-related bHLH transcription factor family. It is required for neurogenesis and can be used as a marker for cells of neuronal lineage.

Entrez Gene IDs

63973 (Human); 11924 (Mouse); 295475 (Rat)

Alternate Names

Atoh4; BHLHA8; bHLHa8MGC46562; Class A basic helix-loop-helix protein 8; Math4A; NEUROG2; neurogenin 2; Neurogenin2; Neurogenin-2; NGN2; ngn-2; Protein atonal homolog 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Rat Neurogenin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors
Authors: Ozkan, A;Padmanabhan, HK;Shipman, SL;Azim, E;Kumar, P;Sadegh, C;Basak, AN;Macklis, JD;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Transfected Whole Cells
Applications: Immunocytochemistry
Directly reprogrammed fragile X syndrome dorsal forebrain precursor cells generate cortical neurons exhibiting impaired neuronal maturation
Authors: Edwards, N;Combrinck, C;McCaughey-Chapman, A;Connor, B;
Frontiers in cellular neuroscience
Species: Human
Sample Types: Whole Cells
Applications: ICC
Hierarchical deployment of Tbx3 dictates the identity of hypothalamic KNDy neurons to control puberty onset
Authors: X Shi, Y Zhuang, Z Chen, M Xu, J Kuang, XL Sun, L Gao, X Kuang, H Zhang, W Li, SZH Wong, C Liu, L Liu, D Jiang, D Pei, Y Lin, QF Wu
Science Advances, 2022-11-16;8(46):eabq2987.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Responses of Epibranchial Placodes to Disruptions of the FGF and BMP Signaling Pathways in Embryonic Mice
Authors: Stefan Washausen, Wolfgang Knabe
Frontiers in Cell and Developmental Biology
Cascade diversification directs generation of neuronal diversity in the hypothalamus
Authors: YH Zhang, M Xu, X Shi, XL Sun, W Mu, H Wu, J Wang, S Li, P Su, L Gong, M He, M Yao, QF Wu
Cell Stem Cell, 2021-04-21;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors
Authors: S Cheng, T Liu, Y Hu, Y Xia, J Hou, C Huang, X Zou, J Liang, Y Stone Shi, Y Zheng, J Lu, G Chen
J. Neurosci., 2019-01-28;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Conversion of adult human fibroblasts into neural precursor cells using chemically modified mRNA
Authors: B Connor, E Firmin, A McCaughey-, R Monk, K Lee, S Liot, J Geiger, C Rudolph, K Jones
Heliyon, 2018-11-08;4(11):e00918.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Requirements for Neurogenin2 during mouse postnatal retinal neurogenesis
Authors: Angelica M. Kowalchuk, Kate A. Maurer, Farnaz Shoja-Taheri, Nadean L. Brown
Developmental Biology
Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals
Authors: Stefan Washausen, Wolfgang Knabe
Biology Open
Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors
Journal of Stem Cells and Regenerative Medicine

BMP/SMAD pathway promotes neurogenesis of midbrain dopaminergic neurons in vivo and in human induced pluripotent and neural stem cells
Authors: VM Jovanovic, A Salti, H Tilleman, K Zega, MM Jukic, H Zou, RH Friedel, N Prakash, S Blaess, F Edenhofer, C Brodski
J. Neurosci., 2018-01-10;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis
Authors: Haoran Zhang, Li Wang, Elaine Yee Man Wong, Sze Lan Tsang, Pin-Xian Xu, Urban Lendahl et al.
eLife

Notch signaling differentially regulates Atoh7 and Neurog2 in the distal mouse retina.
Authors: Maurer K, Riesenberg A, Brown N
Development, 2014-08-01;141(16):3243-54.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Characterization of progenitor domains in the developing mouse thalamus.
Authors: Vue TY, Aaker J, Taniguchi A
J. Comp. Neurol., 2007-11-01;505(1):73-91.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr