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Human/Rat Tollip Antibody

Catalog #: MAB4678
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4678
MAB4678-SP
Detection of Human/Rat Tollip by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Tollip Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat Tollip in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 503315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Tollip
Met1-Pro274
Accession # Q9H0E2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat Tollip antibody by Western Blot. View Larger

Detection of Human/Rat Tollip by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat Tollip Monoclonal Antibody (Catalog # MAB4678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tollip at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tollip

Tollip (Toll-interacting protein) is a 28 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids(aa) in length. It contains a C2 Ca++-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa 6‑33. Human Tollip is 92% identical to mouse Tollip.

Long Name
Toll Interacting Protein
Entrez Gene IDs
54472 (Human); 54473 (Mouse); 361677 (Rat)
Alternate Names
adapter protein; FLJ33531; IL-1RAcPIP; toll interacting protein; toll-interacting protein; Tollip

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Citations for Human/Rat Tollip Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tollip-deficient zebrafish display no abnormalities in development, organ morphology or gene expression in response to lipopolysaccharide
    Authors: L Woli?ska-N, K Romaniuk, K Wojciechow, K Surga, M Kamaszewsk, H Szudrowicz, M Mi?czy?ska
    FEBS Open Bio, 2022-06-27;0(0):.
    Species: Zebrafish
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Endocytic Adaptor Protein Tollip Inhibits Canonical Wnt Signaling.
    Authors: Torun A, Szymanska E, Castanon I, Wolinska-Niziol L, Bartosik A, Jastrzebski K, Mietkowska M, Gonzalez-Gaitan M, Miaczynska M
    PLoS ONE, 2015-06-25;10(6):e0130818.
    Species: Zebrafish
    Sample Types: Whole Organism
    Applications: IHC

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