Human RBX1 Antibody

Detection of Human RBX1 by Western Blot. Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human RBX1 Monoclonal Antibody (Catalog # MAB11499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RBX1 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of RBX1 in Prostate Tumor. RBX1 was detected in immersion fixed paraffin-embedded sections of prostate tumor using Mouse Anti-Human RBX1 Monoclonal Antibody (Catalog # MAB11499) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of RBX1 in MCF-7 cells (Positive) and Jurkat cells (Negative). RBX1 was detected in fixed MCF‑7 human breast cancer cell line (Positive) and absent in Jurkat human acute T cell leukemia cell line (Negative) using Mouse Anti-Human RBX1 Monoclonal Antibody (Catalog # MAB11499) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human RBX1 by Simple Western^TM. Simple Western shows lysates of HEK293T human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for RBX1 at approximately 19 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human RBX1 Monoclonal Antibody (Catalog # MAB11499). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.