Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16‑18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet beta -cells and intestinal villi. It is up‑regulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65%‑68% aa sequence identity with mouse and rat Reg1A.
Human Reg1A Antibody
R&D Systems | Catalog # MAB4937
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 431202
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Product Specifications
Immunogen
E. coli-derived recombinant human Reg1A
Gln23-Asn166
Accession # P05451
Gln23-Asn166
Accession # P05451
Specificity
Detects human Reg1A in Western blots. Shows 5% cross-reactivity with recombinant human (rh) Reg1B and no cross-reactivity with rhReg3A, recombinant mouse Reg1, or recombinant rat Reg1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human Reg1A Antibody
Detection of Reg1A in AGS Human Cell Line by Flow Cytometry.
AGS human gastric adenocarcinoma cell line was stained with Rat Anti-Human Reg1A Monoclonal Antibody (Catalog # MAB4937, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Applications for Human Reg1A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: AGS human gastric adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: AGS human gastric adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human Reg1A
Sample: Recombinant Human Reg1A
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Reg1A
Long Name
Regenerating Islet-derived 1A
Alternate Names
ICRF, PSP, PTP
Entrez Gene IDs
5967 (Human)
Gene Symbol
REG1A
UniProt
Additional Reg1A Products
Product Documents for Human Reg1A Antibody
Product Specific Notices for Human Reg1A Antibody
For research use only
Related Research Areas
Citations for Human Reg1A Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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