Human Relaxin-2 Antibody

Name: Human Relaxin-2 Antibody
Brand: R&D Systems
SKU: MAB2804
Rating: 5 (1 reviews)

Catalog #: MAB2804
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2804
MAB2804-SP

Detection of Human Relaxin-2 by Western Blot.

2 Images

All Relaxin-2 Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Relaxin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects both the pro and mature forms of human Relaxin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Relaxin-1 or rhRelaxin-3 is observed.

Source

Monoclonal Rat IgG₁ Clone # 349102

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Relaxin-2
Val23-Cys185
Accession # P04090

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Neutralization

Measured by its ability to neutralize Relaxin‑2-induced cAMP production in the THP‑1 human acute monocytic leukemia cell line. Parsell, D. A. et al. (1996) J. Biol. Chem. 271:27936. The Neutralization Dose (ND₅₀) is typically 1.5-6.0 µg/mL in the presence of 7.5 ng/mL Recombinant Human Relaxin‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Relaxin-2 by Western Blot. Western blot shows lysates of human placenta. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Relaxin-2 Monoclonal Antibody (Catalog # MAB2804) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Relaxin-2 at approximately 8 kDa and 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization

View Larger

cAMP production Induced by Relaxin‑2 and Neutralization by Human Relaxin‑2 Antibody. Recombinant Human Relaxin-2 (Catalog # 3596-RN) induces cAMP production in the THP-1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line), as measured by cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recombinant Human Relaxin-2 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human Relaxin-2 Monoclonal Antibody (Catalog # MAB2804). The ND₅₀ is typically 1.5-6.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Relaxin-2

Human Relaxin-2/H2 is a 6 kDa member of the insulin/relaxin gene superfamily. Relaxin-2 is synthesized as a 185 aa preprohormone that is proteolytically processed into a nonglycosylated, disulfide-linked, 53 aa mature heterodimer that contains a 29 aa B chain (aa 25‑53) and a 24 aa A chain (aa 161‑185). An alternate splice form of human Relaxin-2 is known that contains the B chain and an uncleaved 47 aa extension. Mature human Relaxin-2 heterodimer is 48%, 44% and 43% aa identical to rat, canine and porcine Relaxin-2, respectively.

Entrez Gene IDs

6019 (Human)

Alternate Names

bA12D24.1.1; bA12D24.1.2; H2; relaxin 2; Relaxin2; Relaxin-2; RLN2; RLXH2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Relaxin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Controlled extracellular matrix degradation in breast cancer tumors improves therapy by trastuzumab.
Authors: Beyer I, Li Z, Persson J, Liu Y, van Rensburg R, Yumul R, Zhang XB, Hung MC, Lieber A
Mol. Ther., 2010-11-16;19(3):479-89.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation