Human Relaxin R2 Antibody

Catalog #: MAB102501 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB102501-100
MAB102501-SP

Detection of Relaxin R2 in HEK293 Human Cell Line Transfected with Human Relaxin R2 and eGFP by Flow Cytometry.

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All Relaxin R2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Relaxin R2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Relaxin R2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1013903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Relaxin R2
Met1-Asn416
Accession # Q8WXD0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Relaxin R2 in HEK293 Human Cell Line Transfected with Human Relaxin R2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Relaxin R2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Relaxin R2 Monoclonal Antibody (Catalog # MAB102501) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Relaxin R2

Relaxin R2 (also known as LGR8) is the G-protein coupled receptor (GPCR) for Relaxin, and may also be a receptor for INSL3. LGR8 can mediate the action of Relaxin through cAMP-dependent pathway distinct from structurally related insulin and insulin-like growth factor family ligand. Distribution of this receptor implicates its role in reproductive, brain, renal, cardiovascular and other functions.

Long Name

Relaxin Receptor 2

Entrez Gene IDs

122042 (Human); 140498 (Mouse)

Alternate Names

G protein coupled receptor affecting testicular descent; Gpr106; GPR106G-protein coupled receptor 106; Great; GREATRelaxin family peptide receptor 2; INSL3R; leucine-rich repeat-containing G protein-coupled receptor 8; Leucine-rich repeat-containing G-protein coupled receptor 8; Lgr8; LGR8.1; LGR8G-protein coupled receptor affecting testicular descent; Relaxin R2; relaxin receptor 2; relaxin/insulin-like family peptide receptor 2; RelaxinR2; RLN2R; Rxfp2; RXFPR2