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Human Rev-erb beta /NR1D2 Antibody

Catalog #: PP-H2729-00
1 Citation Datasheet / COA / SDS
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PP-H2729-00
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Citations (1)
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Human Rev-erb beta /NR1D2 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human Rev-Erb beta and cross-reacts with rat Rev-Erb beta. It does not recognize human Rev-Erb alpha. Not yet tested in other species.
Source
Monoclonal Mouse IgG3 Clone # H2729
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human Rev-erb beta/NR1D2
aa 4-101
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL under reducingconditions with the appropriate secondary reagents to detect human Rev-Erb beta.
 
Immunohistochemistry
This antibody can be used at 50 - 100 μg/mL withthe appropriate secondary reagents to detect human Rev-Erb beta.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.3 μg/mL with the appropriatesecondary reagents to detect human Rev-Erb beta.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Rev-erb beta/NR1D2

Reverse Orientation the c-ErbA-1 Gene beta (Rev-Erb beta, BD73; NR1D2) is a member of the Orphan Nuclear Receptor family. Rev-Erb beta is expressed in the heart, brain, lung, liver, skeletal muscles, kidney, spleen and testis. Rev-Erb beta plays a broad role in cell proliferation and physiology.

Entrez Gene IDs
9975 (Human)
Alternate Names
BD73; EAR-1R; HZF2; NR1D2; nuclear receptor subfamily 1 group D member 2; nuclear receptor subfamily 1, group D, member 2; Orphan nuclear hormone receptor BD73; Reverb beta; Rev-erb beta; rev-erba-alpha-related receptor; rev-erb-beta; RVR; V-erbA-related protein 1-related

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Citation for Human Rev-erb beta /NR1D2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. NR1D2 Accelerates Hepatocellular Carcinoma Progression by Driving the Epithelial-to-Mesenchymal Transition
    Authors: H Tong, X Liu, T Li, W Qiu, C Peng, B Shen, Z Zhu
    Onco Targets Ther, 2020-05-08;13(0):3931-3942.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue, Whole Cells
    Applications: Flow Cytometry, IHC, Western Blot

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