Human RFX6 Antibody

Catalog #: AF7780
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7780
AF7780-SP

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All RFX6 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RFX6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RFX6 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human RFX6
Lys324-Thr511
Accession # Q8HWS3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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RFX6 in Human Pancreas. RFX6 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human RFX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7780) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RFX6

RFX6 (regulatory factor X, member 6) is a 928 amino acid, ~102 kDa (calculated) member of the RFX family of transcription factors that are critical for development. RFX6 is expressed in the gut endoderm, later becoming restricted to developing and adult pancreatic islets. It is downstream of Neurogenin-3 and essential for differentiation of most islet cell types. Mutation in humans can produce autosomal recessive neonatal diabetes. Human RFX6 shares 96% and 95% amino acid sequence identity with mouse and rat RFX6, respectively.

Long Name

Regulatory Factor X, 6

Entrez Gene IDs

222546 (Human); 320995 (Mouse); 294395 (Rat)

Alternate Names

dJ955L16.1; DNA-binding protein RFX6; MGC33442; MTFS; Regulatory factor X 6; Regulatory factor X domain-containing protein 1; regulatory factor X, 6; RFX6; RFXDC1; RFXDC1regulatory factor X domain containing 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human RFX6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Identification of novel, clinically correlated autoantigens in the monogenic autoimmune syndrome APS1 by proteome-wide PhIP-Seq
Authors: SE Vazquez, EM Ferré, DW Scheel, S Sunshine, B Miao, C Mandel-Bre, Z Quandt, AY Chan, M Cheng, M German, M Lionakis, JL DeRisi, MS Anderson
Elife, 2020-05-15;9(0):.
Species: Human
Sample Types: Serum
Applications: Immunoprecipitation