Human RGM-A Antibody Summary
Cys48-Gly422
Accession # Q96B86
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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RGM‑A in Human Brain. RGM-A was detected in immersion fixed paraffin-embedded sections of human brain (spinal cord) using Goat Anti-Human RGM-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2459) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RGM-A
Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (2). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of human RGM-A has 88% and 93% aa identity to the chick and mouse mature region of RGM-A, respectively. When compared to human RGMb and c, the mature region of human RGM-A shows 58% and 54% aa identity, respectively. Recombinant chick RGM-A has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mammals, however, this activity is not so evident, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure, and may play a role in entorhinal-hippocampal connections (3, 4). The receptor for RGM-A is reported to be neogenin (5, 6). RGM-A has also been shown to be a bone morphogenic protein co-receptor, able to bind both BMP-2 and BMP-4 (7).
- Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
- Monnier P. et al. (2002) Nature 419:392.
- Niederkofler V. et al. (2004) J. Neurosci. 24:808.
- Brinks, H. et al. (2004) J. Neurosci. 24:3862.
- Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
- Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
- Babitt J.L. et al. (2005) J. Biol. Chem. 280(33):29820.
Product Datasheets
Citations for Human RGM-A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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MiR-552-3p promotes malignant progression of gallbladder carcinoma by reactivating the Akt/ beta -catenin signaling pathway due to inhibition of the tumor suppressor gene RGMA
Authors: Fengliang Song, Zhao Yang, Liang Li, Yanping Wei, Xuewu Tang, Shuowu Liu et al.
Annals of Translational Medicine
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The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration
Authors: AA Villanueva, P Falcón, N Espinoza, LS R, LA Milla, E Hernandez-, VA Torres, P Sanchez-Go, V Palma
Oncotarget, 2017-02-07;8(6):9767-9782.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Downregulation of the Sonic Hedgehog/Gli pathway transcriptional target Neogenin-1 is associated with basal cell carcinoma aggressiveness
Authors: Bárbara S. Casas, Christelle Adolphe, Pablo Lois, Nelson Navarrete, Natalia Solís, Eva Bustamante et al.
Oncotarget
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