Human RGS8 Antibody

Catalog #: AF6880 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6880
AF6880-SP

Detection of Human RGS8 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RGS8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RGS8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human RGS4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RGS8
Asn10-Thr76
Accession # P57771

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RGS8 by Western Blot. Western blot shows lysates of human brain (Alzheimer's disease cortex) tissue and human brain (normal cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RGS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6880) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RGS8 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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RGS8 in Human Brain. RGS8 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human RGS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6880) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGS8

RGS8 (Regulator of G-protein signaling 8) is a 22-23 kDa (predicted) member of the B/R4 subfamily, RGS family of proteins. It is expressed in NK cells and neurons, particularly cerebellar Purkinje cells, and is known to regulate GPCR signaling. When GPCRs are activated, a GPCR cytoplasmic domain associated G alpha beta gamma heterotrimer dissociates in response to GTP binding. This generates G alpha :GTP and G beta gamma dimers that initiate downstream signaling. G alpha has GTPase activity, and when GTP is hydrolyzed, the G alpha beta gamma trimer reforms and stops signaling. RGS8 potentiates G alpha GTP hydrolase activity, particularly when associated with M1 muscarinic acetylcholine receptors. Human RGS8 is 180 amino acids (aa) in length, and is characterized by the presence of one RGS domain (aa 56-171), and an N-terminal GPCR binding motif (aa 6-9). There is one splice variant that shows a 27 aa substitution for aa 1-9. Expression of this variant may negatively impact its regulation of G alpha -coupled responses. Over aa 10-76, human RGS8 shares 98% aa identity with mouse RGS8.

Long Name

Regulator of G-protein Signaling 8

Entrez Gene IDs

85397 (Human); 67792 (Mouse); 54297 (Rat)

Alternate Names

MGC119067; MGC119068; MGC119069; regulator of G-protein signaling 8; regulator of G-protein signalling 8; RGS8