Human RIPK3/RIP3 Antibody

Name: Human RIPK3/RIP3 Antibody
Brand: R&D Systems
SKU: MAB76042
Rating: 5 (1 reviews)

Catalog #: MAB76042 1 Review Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB76042-100
MAB76042-SP

Detection of Human RIPK3/RIP3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RIPK3/RIP3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RIPK3/RIP3 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1312D

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing human RIPK3/RIP3
Accession # Q9Y572

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RIPK3/RIP3 by Western Blot. Western blot shows lysates of SK-BR-3 human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB76042) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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RIPK3/RIP3 in SK-BR-3 and HepG2 Human Cell Lines. RIPK3/RIP3 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive stain) and HepG2 human hepatocellular carcinoma cell line (negative stain) using Rabbit Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB76042) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIPK3/RIP3

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3

Entrez Gene IDs

11035 (Human); 56532 (Mouse); 246240 (Rat)

Alternate Names

EC 2.7.11.1; Receptor Interacting Serine/Threonine Kinase 3; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine-threonine kinase 3; RIP3; RIP-3; RIPK3; RIP-like protein kinase 3