Human RLK/TXK Antibody

Catalog #: MAB6520 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6520
MAB6520-SP

Detection of Human RLK/TXK by Western Blot.

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All RLK/TXK Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RLK/TXK Antibody Summary

Species Reactivity

Human

Specificity

Detects human RLK/TXK in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 674801

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human RLK/TXK
Trp150-Val246
Accession # P42681

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RLK/TXK by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human primary differentiated Th2 cells. PVDF Membrane was probed with 1 µg/mL of Human RLK/TXK Monoclonal Antibody (Catalog # MAB6520) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RLK/TXK at approximately 58 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RLK/TXK

RLK (resting lymphocyte kinase), also called TXK (T cell-expressed kinase) or PTK4, is a protein tyrosine kinase of the TEK family. It is expressed in T cells and some myeloid cells, especially in Th1 cells in inflammatory diseases such as rheumatoid arthritis and Behcet’s disease. Upon TCR stimulation, it translocates to the nucleus and induces IFN-gamma production. An alternate start site creates a resident nuclear 55 kDa form in addition to the larger (~58 kDa) form that is cytoplasmic or targeted to the plasma membrane via palmitoylation of a cysteine string motif. The region used as an immunogen is common to both forms and shares 79% and 81% amino acid identity with mouse and rat RLK, respectively.

Long Name

Resting Lymphocyte Kinase

Entrez Gene IDs

7294 (Human)

Alternate Names

BTKL; BTKLEC 2.7.10.2; EC 2.7.10; MGC22473; Protein-tyrosine kinase 4; PSCTK5; PTK4 protein tyrosine kinase 4; PTK4; RLK; TKL; TXK tyrosine kinase; TXK; tyrosine kinase; tyrosine-protein kinase TXK