Home / ROBO2 / Human ROBO2 Antibody

Human ROBO2 Antibody

Catalog #: AF3147
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3147
AF3147-SP
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO2
Ser22-Ala312, RPVA, Pro313-Pro859
Accession # Q9HCK4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB)
Flow Cytometry
2.5 µg/106 cells
NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.

Long Name
Roundabout Homolog 2
Entrez Gene IDs
6092 (Human); 268902 (Mouse); 84409 (Rat)
Alternate Names
Drosophila) homolog 2; ROBO2; roundabout, axon guidance receptor, homolog 2 (Drosophila); SAX3

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human ROBO2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Evolution of Cortical Neurogenesis in Amniotes Controlled by Robo Signaling Levels
    Authors: Adrián Cárdenas, Ana Villalba, Camino de Juan Romero, Esther Picó, Christina Kyrousi, Athanasia C. Tzika et al.
    Cell
  2. Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin
    Authors: Maud Wurmser, Mridula Muppavarapu, Christine Mary Tait, Christophe Laumonnerie, Luz María González-Castrillón, Sara Ivy Wilson
    Frontiers in Cell and Developmental Biology
  3. Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
    Authors: M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw
    Cell Rep, 2019-03-19;26(12):3298-3312.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  4. The chemorepellent Slit3 promotes monocyte migration.
    Authors: Geutskens SB, Hordijk PL, van Hennik PB
    J. Immunol., 2010-11-15;185(12):7691-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
    Authors: Takeuchi H, Inokuchi K, Aoki M
    Cell, 2010-06-11;141(6):1056-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
    Authors: Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z
    J. Neurosci., 2008-08-27;28(35):8698-708.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons.
    Authors: Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chedotal A
    J. Neurosci., 2008-04-16;28(16):4244-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.
    Authors: Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG
    Dev. Biol., 2007-11-13;313(2):648-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons
    Authors: Max Koppers, Roberta Cagnetta, Toshiaki Shigeoka, Lucia CS Wunderlich, Pedro Vallejo-Ramirez, Julie Qiaojin Lin et al.
    eLife
  10. Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells
    Authors: Pooja Hor, Justin K. Ichida, Zea Borok, Amy L. Ryan
    STAR Protocols

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human ROBO2 Antibody

There are currently no reviews for this product. Be the first to review Human ROBO2 Antibody and earn rewards!

Have you used Human ROBO2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review