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Human ROBO4 Antibody

Catalog #: AF2366
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2366
AF2366-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROBO4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO2, and rhROBO3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO4
Gln28-Arg467
Accession # Q8WZ75
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ROBO4
Flow Cytometry
0.25 µg/106 cells
HUVEC human umbilical vein endothelial cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO4

ROBO4 belongs to the ROBO family, which are molecular guidance receptors that interact with Slit ligand to regulate axon guidance, neuronal migration and leukocyte chemotaxis. The expression of ROBO4 is restricted in the endothelium, notably in areas of angiogenesis.

Long Name
Roundabout Homolog 4
Entrez Gene IDs
54538 (Human); 74144 (Mouse)
Alternate Names
ECSM4; FLJ20798; Magic roundabout; MGC133352; MGC133353; MRB; ROBO4; roundabout homolog 4; roundabout homolog 4, magic roundabout (Drosophila)

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Citations for Human ROBO4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Robo 4 - the double-edged sword in prostate cancer: impact on cancer cell aggressiveness and tumor vasculature
    Authors: A Pircher, G Schäfer, A Eigentler, R Pichler, M Puhr, E Steiner, W Horninger, E Gunsilius, H Klocker, I Heidegger
    Int J Med Sci, 2019-01-01;16(1):115-124.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. The Robo4-TRAF7 complex suppresses endothelial hyperpermeability in inflammation
    Authors: K Shirakura, R Ishiba, T Kashio, R Funatsu, T Tanaka, SI Fukada, K Ishimoto, N Hino, M Kondoh, Y Ago, Y Fujio, K Yano, T Doi, WC Aird, Y Okada
    J. Cell. Sci., 2019-01-02;0(0):.

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