Human ROR1 Antibody

Catalog #: MAB20001 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB20001-100
MAB20001-SP

Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.

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All ROR1 Antibodies

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Human ROR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ROR1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1011919

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ROR1
Ala24-Glu403
Accession # Q01973

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human ROR1 Monoclonal Antibody (Catalog # MAB20001, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR1

ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 1

Entrez Gene IDs

4919 (Human); 26563 (Mouse)

Alternate Names

EC 2.7.10.1; MGC99659; neurotrophic tyrosine kinase receptor-related 1; Neurotrophic tyrosine kinase, receptor-related 1; NTRKR1; NTRKR1dJ537F10.1; receptor tyrosine kinase-like orphan receptor 1; ROR1; tyrosine-protein kinase transmembrane receptor ROR1