Human RUNX1/CBFA2 Antibody

Detection of Human RUNX1/CBFA2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RUNX1/CBFA2 in K562 Human Cell Line. RUNX1/CBFA2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Porcine RUNX1/CBFA2 by Western Blot RUNX1 enhances TGEV-induced apoptosis.(A) Silencing effect of RUNX1 siRNAs on RUNX1 at mRNA level. PK-15 cells were transfected with RUNX1-specific siRNA or irrelevant siRNA and measured by real-time PCR (normalized to beta -actin). (B) The silencing effect of siRUNX1-2 on RUNX1 expression. (C) The effect of siRUNX1-2 on PK-15-cell viability. The cells were incubated after transfecting with 100 nM siRUNX1-2 for 48 h. Cell viability was evaluated by CCK-8 assay. (D) The over-expression of RUNX1 using pCI-neo-RUNX1. PK-15 cells were transfected with pCI-neo- RUNX1 or pCI-neo vector, and the expression level of RUNX1 was assessed by western blot at 48 hpt. (E) The effect of RUNX1 on the expression of Bax. (F) The effect of RUNX1 on caspase-9 activity. (G) The effect of RUNX1 on caspase-3 activity. Data represent mean ± S.D. of three independent experiments. ∗P < 0.05 in comparison with the control. ∗∗P < 0.01 in comparison with the control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26870610), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Porcine RUNX1/CBFA2 by Western Blot RUNX1 enhances TGEV-induced apoptosis.(A) Silencing effect of RUNX1 siRNAs on RUNX1 at mRNA level. PK-15 cells were transfected with RUNX1-specific siRNA or irrelevant siRNA and measured by real-time PCR (normalized to beta -actin). (B) The silencing effect of siRUNX1-2 on RUNX1 expression. (C) The effect of siRUNX1-2 on PK-15-cell viability. The cells were incubated after transfecting with 100 nM siRUNX1-2 for 48 h. Cell viability was evaluated by CCK-8 assay. (D) The over-expression of RUNX1 using pCI-neo-RUNX1. PK-15 cells were transfected with pCI-neo- RUNX1 or pCI-neo vector, and the expression level of RUNX1 was assessed by western blot at 48 hpt. (E) The effect of RUNX1 on the expression of Bax. (F) The effect of RUNX1 on caspase-9 activity. (G) The effect of RUNX1 on caspase-3 activity. Data represent mean ± S.D. of three independent experiments. ∗P < 0.05 in comparison with the control. ∗∗P < 0.01 in comparison with the control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26870610), licensed under a CC-BY license. Not internally tested by R&D Systems.