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Human RUNX2/CBFA1 Antibody

Catalog #: AF2006
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2006
AF2006-SP
Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation.
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Citations (13)
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Human RUNX2/CBFA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RUNX2/CBFA1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) RUNX1 and rhRUNX3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys219-Tyr404
Accession # Q13950
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human RUNX2/CBFA1
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed MC3T3-E1 mouse preosteoblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Chromatin Immunoprecipitation (ChIP) Detection of RUNX2/CBFA1-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. RUNX2/CBFA1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human RUNX2/CBFA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2006) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheVEGFpromoter was detected by standard PCR.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX2/CBFA1

Core Binding Factor A1 (CBFA1; also called AML-3, PEBP-2 alpha A, OSF-2, RUNX2) is a 56 kDa DNA-binding protein that belongs to the Runt-related family of transcription factors. It is synthesized as a 521 amino acid (aa) protein with multiple domains. From the N-terminus, there is a 19 aa activation domain (AD1), a 41 aa glutamine/alanine (Q/A) repeat segment (AD2), a 128 aa Runt/DNA-binding region, a 9 aa NLS and a 285 aa ProSerThr (PST)-rich sequence that is needed for gene activation. Two isoforms exist, one with a truncated AD1 domain and another with a 22 aa deletion in the AD3 section of PST-rich sequence. Human CBFA1 is 98% aa identical to mouse CBFA1.

Long Name
Runt-related Transcription Factor 2
Entrez Gene IDs
860 (Human)
Alternate Names
Acute myeloid leukemia 3 protein; CBFA1; CBF-alpha-1; CCD1; CCDAML3; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120023; ML3; oncogene AML-3; OSF2; OSF-2; osteoblast-specific transcription factor 2; PEA2aA; PEA2-alpha A; PEBP2A; PEBP2aA; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit

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Citations for Human RUNX2/CBFA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. RUNX2 correlates with subtype-specific breast cancer in a human tissue microarray, and ectopic expression of Runx2 perturbs differentiation in the mouse mammary gland.
    Authors: McDonald L, Ferrari N, Terry A et al.
    Dis Model Mech
  2. miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition
    Authors: Liangcong Hu, Jing Liu, Hang Xue, Adriana C. Panayi, Xudong Xie, Ze Lin et al.
    Molecular Therapy - Nucleic Acids
  3. Mesenchymal Wnts are required for morphogenetic movements of calvarial osteoblasts during apical expansion
    Authors: Polsani, N;Yung, T;Thomas, E;Phung-Rojas, M;Gupta, I;Denker, J;Feng, X;Ibarra, B;Hopyan, S;Atit, RP;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes
    Authors: M Minguzzi, V Panichi, S D'Adamo, S Cetrullo, L Cattini, F Flamigni, E Mariani, RM Borzì
    International Journal of Molecular Sciences, 2021-11-05;22(21):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Physiologic Mechanical Stress Directly Induces Bone Formation by Activating Glucose Transporter 1 (Glut 1) in Osteoblasts, Inducing Signaling via NAD+-Dependent Deacetylase (Sirtuin 1) and Runt-Related Transcription Factor 2 (Runx2)
    Authors: S Somemura, T Kumai, K Yatabe, C Sasaki, H Fujiya, H Niki, K Yudoh
    International Journal of Molecular Sciences, 2021-08-23;22(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Ascorbic Acid Promotes KIR Demethylation during Early NK Cell Differentiation
    Authors: Cheng-Ying Wu, Bin Zhang, Hansol Kim, Stephen K. Anderson, Jeffrey S. Miller, Frank Cichocki
    The Journal of Immunology
  7. An Investigation Into the Role of Osteocalcin in Human Arterial Smooth Muscle Cell Calcification
    Authors: Sophie A. Millar, Stephen G. John, Christopher W. McIntyre, Vera Ralevic, Susan I. Anderson, Saoirse E. O'Sullivan
    Front Endocrinol (Lausanne)
  8. RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN
    Authors: Valentina Sancisi, Gloria Manzotti, Mila Gugnoni, Teresa Rossi, Greta Gandolfi, Giulia Gobbi et al.
    Nucleic Acids Research
  9. Icaritin, a novel plant-derived osteoinductive agent, enhances the osteogenic differentiation of human bone marrow- and human adipose tissue-derived mesenchymal stem cells
    Authors: Tao Wu, Tao Shu, Le Kang, Jinhui Wu, Jianzhou Xing, Zhiqin Lu et al.
    International Journal of Molecular Medicine
  10. FGF-2 induces the proliferation of human periodontal ligament cells and modulates their osteoblastic phenotype by affecting Runx2 expression in the presence and absence of osteogenic inducers
    Authors: SHAOFENG AN, XIANGYA HUANG, YAN GAO, JUNQI LING, YIHUA HUANG, YIN XIAO
    International Journal of Molecular Medicine
  11. Histone Deacetylase Inhibitors Repress Tumoral Expression of the Proinvasive Factor RUNX2.
    Authors: Sancisi V, Gandolfi G, Ambrosetti D, Ciarrocchi A
    Cancer Res, 2015-03-13;75(9):1868-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes.
    Authors: Olivotto E, Borzi RM, Vitellozzi R, Pagani S, Facchini A, Battistelli M, Penzo M, Li X, Flamigni F, Li J, Falcieri E, Facchini A, Marcu KB
    Arthritis Rheum., 2008-01-01;58(1):227-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cues
    Authors: Xiaotian Feng, Helen Molteni, Megan Gregory, Jennifer Lanza, Nikaya Polsani, Rachel Wyetzner et al.
    bioRxiv

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