Human S1P3/EDG-3 Antibody

Catalog #: MAB7524 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7524
MAB7524-SP

Detection of S1P3/EDG-3 in S1P3/EDG-3 and eGFP Co-Transfected Cells by Flow Cytometry.

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All S1P3/EDG-3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S1P3/EDG-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human S1P₃/EDG‑3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 776808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human S1P3/EDG-3 transfected NS0 cells

Accession # Q99500

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of S1P₃/EDG-3 in S1P₃/EDG-3 and eGFP Co-Transfected Cells by Flow Cytometry. Human S1P₃/EDG-3 and eGFP co-transfectants were stained with either (A) Mouse IgG2A Isotype Control (Catalog # MAB003) or (B) Mouse Anti-Human S1P₃/EDG-3 Monoclonal Antibody (Catalog # MAB7524) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P3/EDG-3

Sphingosine 1-Phosphate receptor-3 (S1P3), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P3 show 74% aa identity with mouse and rat S1P3.

Long Name

Endothelial Differentiation Gene 3

Entrez Gene IDs

1903 (Human); 13610 (Mouse); 306792 (Rat)

Alternate Names

EDG3; EDG-3; EDG3FLJ37523; Endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; FLJ93220; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; MGC71696; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; S1PR3; sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3; sphingosine-1-phosphate receptor 3