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Human SALL1 Antibody

Catalog #: PP-K9814-00
12 Citations Datasheet / COA / SDS
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PP-K9814-00
Detection of Human SALL1 by Immunocytochemistry/Immunofluorescence
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Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SALL1 Antibody Summary

Species Reactivity
Human
Source
Monoclonal Mouse IgG2a Clone # K9814
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Please refer to the PDF datasheet for specifications.
Immunohistochemistry
Please refer to the PDF datasheet for specifications.
Immunoprecipitation
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Human SALL1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human SALL1 by Immunocytochemistry/Immunofluorescence Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) and IM marker Osr1. (c) IM and MM marker expression as Wt1, Pax8, Pax2, Six2 and Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 and proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, and cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: SALL1

SALL1 (Sall-like Protein 1) is a member of the zinc finger Spalt-like protein family. It acts as a potential transcription repressor and may play a role in kidney development. Mutations have been shown to cause Townes-Brocks syndrome, an inherited disorder characterized by multiple birth defects including renal, ear, anal, and limb abnormalities.

Long Name
Sal-like 1
Entrez Gene IDs
6299 (Human)
Alternate Names
HSAL1; sal (Drosophila)-like 1; SAL1; Sal-1; SALL1; sal-like 1 (Drosophila); sal-like protein 1; Spalt-like transcription factor 1; Zinc finger protein 794; Zinc finger protein SALL1; Zinc finger protein Spalt-1; ZNF794TBS

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Citations for Human SALL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids
    Authors: Huang B, Zeng Z, Li H et al.
    bioRxiv
  2. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids
    Authors: Huang B, Zeng Z, Li H et al.
    bioRxiv
  3. ATP/ADP biosensor organoids for drug nephrotoxicity assessment
    Authors: Susa K, Kobayashi K, Galichon P et al.
    Frontiers in cell and developmental biology
  4. SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes
    Authors: Immacolata Giordano, Lucia Pirone, Veronica Muratore, Eukene Landaluze, Coralia Pérez, Valerie Lang et al.
    Frontiers in Cell and Developmental Biology
  5. Spatial Transcriptional Mapping of the Human Nephrogenic Program
    Authors: Lindstrom NO, Sealfon R, Chen X et al.
    Dev Cell
  6. The embryonic ontogeny of the gonadal somatic cells in mice and monkeys
    Authors: K Sasaki, A Oguchi, K Cheng, Y Murakawa, I Okamoto, H Ohta, Y Yabuta, C Iwatani, H Tsuchiya, T Yamamoto, Y Seita, M Saitou
    Cell Reports, 2021-05-04;35(5):109075.
    Species: Cynomolgus Monkey
    Sample Types: Whole Tissue
    Applications: IHC
  7. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network
    Authors: JH Low, P Li, EGY Chew, B Zhou, K Suzuki, T Zhang, MM Lian, M Liu, E Aizawa, C Rodriguez, KSM Yong, Q Chen, JM Campistol, M Fang, CC Khor, JN Foo, JC Izpisua Be, Y Xia
    Cell Stem Cell, 2019-07-11;0(0):.
  8. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
    Authors: Garreta E, Prado P, Tarantino C et al.
    Nature Materials
  9. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
    Authors: Garreta E, Prado P, Tarantino C et al.
    Nature Materials
  10. Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning
    Authors: Nils O. Lindström, Tracy Tran, Jinjin Guo, Elisabeth Rutledge, Riana K. Parvez, Matthew E. Thornton et al.
    Journal of the American Society of Nephrology
  11. Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injury.
    Authors: Imberti, Barbara, Tomasoni, Susanna, Ciampi, Osele, Pezzotta, Anna, Derosas, Manuela, Xinaris, Christod, Rizzo, Paola, Papadimou, Evangeli, Novelli, Rubina, Benigni, Ariela, Remuzzi, Giuseppe, Morigi, Marina
    Sci Rep, 2015-03-06;5(0):8826.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  12. Sall1 regulates embryonic stem cell differentiation in association with nanog.
    Authors: Karantzali E, Lekakis V, Ioannou M
    J. Biol. Chem., 2010-11-09;286(2):1037-45.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP

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