Human SAMHD1 Antibody

Catalog #: MAB8120 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8120
MAB8120-SP

Detection of Human SAMHD1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SAMHD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SAMHD1 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 883335

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SAMHD1
His206-Arg339
Accession # Q9Y3Z3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SAMHD1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SAMHD1 Monoclonal Antibody (Catalog # MAB8120) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SAMHD1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human SAMHD1 by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for SAMHD1 at approximately 70 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human SAMHD1 Monoclonal Antibody (Catalog # MAB8120). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SAMHD1

Human SAM (Sterile Alpha Motif) domain and HD (His/Asp motif-containing phosphohydrolase) domain-containing 1 (SAMHD1), also known as DCIP (dendritic cell IFN-g-induced protein) or MOP5 (monocyte protein 5) is a 70-80 kDa, 626 amino acid (aa) nuclear protein that acts as a dGTP-regulated deoxynucleotide triphosphohydrolase. It restricts HIV replication by binding nucleic acids. SAMHD-1 expression is induced by TNF-a in myeloid lineage cells and lung fibroblasts. Human SAMHD1 mutations are linked to Aicardi-Goutieres Syndrome, which is characterized by symptoms mimicking viral infection. Human SAMHD1 isoforms of 602, 591, and 556 aa lack aa 113-136, 502-536, and 285-354, respectively. The 591 and 556 aa forms are catalytically inactive. Within the region used as an immunogen, human SAMHD1 shares 85% aa sequence identity with mouse and rat SAMHD1.

Long Name

SAM Domain and HD Domain 1

Entrez Gene IDs

25939 (Human); 56045 (Mouse); 311580 (Rat)

Alternate Names

AGS5; CHBL2; DCIP; DCIPMg11; Dendritic cell-derived IFNG-induced protein; EC 3.1.4.-; HDDC1; Monocyte protein 5; MOP5; MOP-5; MOP-5AGS5; SAM domain and HD domain 1; SAM domain and HD domain-containing protein 1; SAMHD1; SBBI88