Human SCARA5 Antibody

Catalog #: MAB4900 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4900
MAB4900-SP

Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry.

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All SCARA5 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SCARA5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SCARA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SCARA5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 673527

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SCARA5
Arg83-His495
Accession # Q6ZMJ2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human SCARA5 Monoclonal Antibody (Catalog # MAB4900, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCARA5

Scavenger receptor A5 (SCARA5) is also known as testis expressed scavenger receptor (Tesr). Class A scavenger receptors, including CL-P1, MARCO, SCARA3, and SR-A1, are type II transmembrane proteins that contain a collagenous stalk (1-3). Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) domain (4-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. It shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).

References