Human SCGF/CLEC11a Antibody

Catalog #: MAB19041 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19041-100
MAB19041-SP

Detection of SCGF/CLEC11a in Lung Cancer.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SCGF/CLEC11a Antibody Summary

Specificity

Detects human SCGF/CLEC11a in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 239004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SCGF/CLEC11a
Gly19-Phe245
Accession # BAA21499

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Lung Cancer.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of SCGF/CLEC11a in Lung Cancer. SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of Lung Cancer using Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB19041) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCGF/CLEC11a

Stem Cell Growth Factor (SCGF) is a secreted sulfated glycoprotein that belongs to the C-type lectin superfamily (CLEC11a) and is expressed primarily in hematopoietic tissues. It functions as a growth factor for primitive hematopoietic progenitor cells. SCGF-beta is a truncated variant of SCGF-alpha that has aa 78 amino acid deletion and lacks a functional calcium-dependent carbohydrate-recognition domain.

Long Name

Stem Cell Growth Factor/C-type Lectin Domain Family 11, Member A

Entrez Gene IDs

6320 (Human); 20256 (Mouse); 29313 (Rat)

Alternate Names

CLEC11a; CLECSF3; LSLCL; P47; SCGF