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Human SDNSF/MCFD2 Antibody

Catalog #: MAB2357
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2357
MAB2357-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SDNSF/MCFD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SDNSF/MCFD2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SDNSF is observed. In Western blots, no cross-reactivity with recombinant mouse SDNSF is observed.
Source
Monoclonal Mouse IgG2B Clone # 294301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SDNSF/MCFD2
Glu27-Gln146
Accession # Q8NI22
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SDNSF/MCFD2 under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (Multiple Coagulation Factor Deficiency 2), was first identified as a secreted molecule that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of a complex that transports proteins from the ER to the Golgi.

Long Name
Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor
Entrez Gene IDs
90411 (Human); 193813 (Mouse)
Alternate Names
DKFZp686G21263; F5F8D2; LMAN1IP; MCFD2; multiple coagulation factor deficiency 2; multiple coagulation factor deficiency protein 2; neural stem cell derived neuronal survival protein; Neural stem cell-derived neuronal survival protein; SDNSF; SDNSFF5F8D

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Citation for Human SDNSF/MCFD2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor
    Authors: H Yagi, M Yagi-Utsum, R Honda, Y Ohta, T Saito, M Nishio, S Ninagawa, K Suzuki, T Anzai, Y Kamiya, K Aoki, M Nakanishi, T Satoh, K Kato
    Nat Commun, 2020-03-13;11(1):1368.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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