Human SEC13 Antibody Summary
Gly123-Gln322
Accession # P55735
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of SEC13 by Western Blot. Western blot shows lysates of human kidney tissue, human testis tissue, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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SEC13 in HeLa Human Cell Line. SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Monoclonal Antibody (Catalog # MAB9055) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SEC13
Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport. Presenillin-1, which is implicated in early-onset Alzheimer's disease has been shown to interact with Sec13 vesicles. Additionally, Sec13 has been shown to be a component of the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the GATOR2 sub-complex of the GATOR complex.
Product Datasheets
Citations for Human SEC13 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Bi-allelic mutation in SEC16B alters collagen trafficking and increases ER stress
Authors: A El-Gazzar, B Voraberger, F Rauch, M Mairhofer, K Schmidt, B Guillemyn, G Mitulovi?, V Reiterer, M Haun, MM Mayr, JA Mayr, S Kimeswenge, O Drews, V Saraff, N Shaw, N Fratzl-Zel, S Symoens, H Farhan, W Högler
Embo Molecular Medicine, 2023-03-14;0(0):e16834.
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COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers
Authors: Olga Shomron, Inbar Nevo-Yassaf, Tamar Aviad, Yakey Yaffe, Eitan Erez Zahavi, Anna Dukhovny et al.
Journal of Cell Biology
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Fibroblasts were fixed with 0.4% paraformaldehyde, washed with DPBS and then permeabilized with 0.2% Triton-X/DPBS, washed and blocked with donkey serum. Sec13 diluted 1:100 and applied to the cells for 90 min, unbound antibody removed with DPBS, washed, then donkey-anti-rabb (1:100) applied for 40 mins. Cells were washed, mounted with DapI and viewed with an epi-fluourescent microscope.
