Human Semaphorin 4G Antibody

Catalog #: AF5840 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5840
AF5840-SP

Detection of Human Semaphorin 4G by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 4G Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 4G in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse (rm) Semaphorin 4G is observed and less than 1% cross reactivity with recombinant human (rh) Semaphorin 3B, rhSemaphorin 4A, and rhSemaphorin 4C is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4G
Val18-Leu675
Accession # Q9NTN9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Perfusion-fixed frozen sections of mouse neural tube

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Semaphorin 4G by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Semaphorin 4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5840) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4G at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4G

Semaphorin 4G (Sema4G) is the least characterized of the seven known Class 4 transmembrane semaphorin glycoproteins (1). Class 4 semaphorins have multiple roles in cell attraction or repulsion. These include development of nerve pathways in the brain, promoting or inhibiting proliferation, and in some cases organizing immune cell interactions. Receptors include transmembrane plexins, but alternate receptors, such as CD72 for Sema4D and TIM-2 for Sema4A, have been identified (2, 3). The 838 amino acid (aa) human Sema4G precursor contains a 17 aa signal sequence, a 658 aa extracellular domain (ECD) containing sema and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 142 aa cytoplasmic domain with one Ser/Thr phosphorylation site (4). The human Sema4G ECD shares 90%, 92%, 93%, 93% and 92% aa identity with mouse, rat, equine, bovine and canine Sema4G, respectively. It also shares 42% aa identity with Sema4C, the most closely related semaphorin. Two additional isoforms have been reported: an 843 aa form contains a 5 aa insert at aa 542, while a 458 aa form diverges at aa 450 near the end of the Sema domain (5). Sema4G mRNA is expressed early in development in the central and peripheral nervous systems and in sensory organs such as retina, cochlea, vomeronasal organ and olfactory epithelium. In adults, Sema4G mRNA is found in predominantly in the liver, but is also detected in the kidneys and brain. Elevated Sema4G mRNA expression was found in mouse kidney during Plasmodium yoelii infection (6). Sema4G was also identified in a screen for genes that are downregulated in human colorectal cancer (7).

References

Li, H. et al. (1999) Mech. Dev. 87:169.
Halloran, M.C. and M.A. Wolman (2006) Curr. Opin. Cell Biol. 18:533.
Suzuki, K. et al. (2008) Nat. Immunol. 9:17.
Swissprot Accession # Q9NTN9.
Entrez Accession # EAW49803, EAW49802.
Lao, A.O.T. et al. (2001) J. Immunol. 166:1945.
Wang, X. et al. (2008) Hepatogastroenterology 55:2039.

Entrez Gene IDs

57715 (Human); 26456 (Mouse); 361764 (Rat)

Alternate Names

KIAA1619FLJ20590; MGC102867; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4G; SEMA4G; Semaphorin 4G; semaphorin-4G