Human Semaphorin 5A Antibody

Catalog #: MAB5896
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5896
MAB5896-SP

Semaphorin 5A in Human Pancreatic Cancer Tissue.

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Citations (1)

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Human Semaphorin 5A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 5A in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 914419

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Semaphorin 5A
Glu23-Thr765
Accession # Q13591

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Semaphorin 5A in Human Pancreatic Cancer Tissue. Semaphorin 5A was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human Semaphorin 5A Monoclonal Antibody (Catalog # MAB5896) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 5A

Semaphorin 5A (Sema5A, previously called SemaF) is a 140 kDa protein of the semaphorin family of axon guidance molecules (1‑4). Class 5 semaphorins are type I transmembrane glycoproteins with an N-terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains
(1‑3). Sema5A is expressed developmentally in oligodendrocytes, neuroepithelial cells surrounding retinal axons, the base of limb buds, the cardiac atrial septum and endocardial cushions, and the mesoderm surrounding cranial vessels (4‑6). The human Sema5A cDNA encodes a 22 amino acid (aa) signal sequence, a 946 aa extracellular domain (ECD), a 22 aa transmembrane sequence and an 85 aa cytoplasmic portion. Within aa 23‑765, which includes the sema domain and four of the seven TSP repeats, human Sema5A shares 93% aa identity with corresponding mouse, rat, and canine sequences. Semaphorins typically transduce signals through transmembrane plexins (1, 2). The sema domain of Sema5A binds plexin B3, triggering signaling via HGF R/c-Met (7). Both Sema5A and plexin B3 are expressed postnatally during differentiation and migration of central nervous system oligodendrocytes. However, plexin B3 is not significantly expressed prenatally and therefore unlikely to be the Sema5A receptor during development (7, 8). The Sema5A TSP repeats interact with either heparin sulfate or chondroitin sulfate proteoglycans (HSPG, CSPG) (9). HSPG interaction promotes attraction, while CSPG interaction promotes repulsion and is essential for axon fasciculation, independent of plexin B3 (9, 10). Sema5A mutations have been implicated in the genetic syndrome, cri-du-chat, while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson’s (3, 11). Sema5A expression may be upregulated in metastatic cancer cells and downregulated in autism (12, 13).

References

Flannery, E. and M. Duman-Scheel (2009) Curr. Drug Targets 10:611.
Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
Simmons, A.D. et al. (1998) Biochem. Biophys. Res. Commun. 242:685.
Oster, S.F. et al. (2003) Development 130:775.
Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.
Artigiani, S. et al. (2004) EMBO Rep. 5:710.
Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
Kantor, D.B. et al. (2004) Neuron 44:961.
Hilario, J.D. et al. (2008) Dev. Biol. 326:190.
Lin, L. et al. (2009) Trends Neurosci. 32:142.
Pan, G-Q. et al. (2009) World J. Gastroenterol. 15:2800.
Melin, M. et al. (2006) Neuropsychobiology 54:64.

Entrez Gene IDs

9037 (Human); 20356 (Mouse); 310207 (Rat)

Alternate Names

FLJ12815; sema domain, seven thrombospondin repeats (type 1 and type 1-like); Sema F; SEMA5A; SEMAF; Semaphorin 5A; semaphorin F; semaphorin-5A; Semaphorin-F; semF; transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

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Citation for Human Semaphorin 5A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A
Authors: M Lobna, E Nasren, S Adi, B Jacob, GA Maria, M Marcus, V Zahava
Biomolecules, 2021-03-02;11(3):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC