Human Semaphorin 7A APC-conjugated Antibody Summary
His47-Ala648
Accession # O75326
Customers also Viewed
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Semaphorin 7A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human Semaphorin 7A APC-conjugated Monoclonal Antibody (Catalog # FAB20681A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 7A
Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1-4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1-5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2-type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6-10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9-12). T cell-expressed Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13).
- Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
- Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
- Sato, Y. and Takahashi H. (1998) Biochim. Biophys. Acta. 1443:419.
- Yamada, A. et al. (1999) J. Immunol. 162:4094.
- Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
- Suzuki, K. et al. (2007) Nature 446:680.
- Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
- Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
- Czopik, A.K. et al. (2006) Immunity 24:591.
- Pasterkamp, R.J. et al. (2003) Nature 424:398.
- Mine, T. et al. (2000) Tissue Antigens 55:429.
- Kang, H.R. et al. (2007) J. Exp. Med. 204:1083.
- Delorme, G. et al. (2005) Biol. Cell 97:589.
Product Datasheets
Citations for Human Semaphorin 7A APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Pyruvate metabolism controls chromatin remodeling during CD4+ T�cell activation
Authors: Mocholi, E;Russo, L;Gopal, K;Ramstead, AG;Hochrein, SM;Vos, HR;Geeven, G;Adegoke, AO;Hoekstra, A;van Es, RM;Pittol, JR;Vastert, S;Rutter, J;Radstake, T;van Loosdregt, J;Berkers, C;Mokry, M;Anderson, CC;O'Connell, RM;Vaeth, M;Ussher, J;Burgering, BMT;Coffer, PJ;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures
Authors: L Man, N Lustgarten, E Kallinos, Z Redhead-La, S Liu, B Schattman, D Redmond, K Hancock, N Zaninovic, G Schattman, Z Rosenwaks, D James
Cell Rep, 2020-08-11;32(6):108027.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC -
Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb
Authors: D Köhler, T Granja, J Volz, M Koeppen, HF Langer, G Hansmann, E Legchenko, T Geisler, T Bakchoul, C Eggstein, HA Häberle, B Nieswandt, P Rosenberge
Nat Commun, 2020-03-11;11(1):1315.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Staining Reagents
Reviews for Human Semaphorin 7A APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human Semaphorin 7A APC-conjugated Antibody and earn rewards!
Have you used Human Semaphorin 7A APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
