Human Semaphorin 7A Biotinylated Antibody

Catalog #: BAF2068 Datasheet / COA / SDS

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BAF2068

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 7A Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 7A in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Semaphorin 7A is observed and less than 1% cross-reactivity with recombinant human Semaphorin 3A, 6B, 6D and rmSema 3C, 3E, and 3F is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

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Citations (5)

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Cat # 10970-NP

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Semaphorin 7A Fc Chimera (Catalog # 2068-S7)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 7A

Semaphorin 7A (Sema 7A), also known as Sema L, Sema K1 and CD108, is an 80 kDa glycosylphosphatidylinositol-anchored protein belonging to the Semaphorin superfamily. It binds to the beta 1 integrin subunit as well as Plexin C1. The Sema domain of human Sema 7A shares 92% and 90% amino acid sequence homology with that of the mouse and canine protein.

Entrez Gene IDs

8482 (Human); 20361 (Mouse)

Alternate Names

CD108 antigen; CD108; CD108MGC126696; CDW108; H-SEMA-K1; H-Sema-L; JMH blood group antigen; JMH; John-Milton-Hargen human blood group Ag; MGC126692; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; Sema K1; Sema L; Sema7A; SEMAK1; SEMAL; SEMALJohn Milton Hagen blood group H-Sema K1; Semaphorin 7A; semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin K1; semaphorin L; semaphorin-7A; Semaphorin-K1; semaphorin-L