Human Serpin A1/ alpha 1-Antitrypsin Antibody

Catalog #: MAB12681 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB12681-100
MAB12681-SP

Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin A1/ alpha 1-Antitrypsin Antibody Summary

Species Reactivity

Human

Specificity

Detects human SerpinA1/ alpha 1-Antitrypsin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1019625

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human Serpin A1/alpha 1-Antitrypsin
Glu25-Lys418
Accession # P01009

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

Human plasma

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, HT-29 human colon adenocarcinoma cell line, and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin A1/a1-Antitrypsin Monoclonal Antibody (Catalog # MAB12681) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Serpin A1/a1-Antitrypsin at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Serpin A1/a1‑Antitrypsin by Simple Western^TM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/a1‑Antitrypsin at approximately 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Serpin A1/a1‑Antitrypsin Monoclonal Antibody (Catalog # MAB12681). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A1/alpha 1-Antitrypsin

Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring Serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe Serpin A1 deficiency (3).

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Barbour, K.W. et al. (2002) Genomics 80:515.
Lomas, D.A. et al. (2002) Biochem. Soc. Trans. 30:89.

Entrez Gene IDs

5265 (Human)

Alternate Names

A1A; A1AT; AATMGC23330; alpha 1-Antitrypsin; alpha 1-Proteinase Inhibitor; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha-1-antitrypsin; antitrypsin), member 1; member 1; PIMGC9222; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; serine (or cysteine) proteinase inhibitor, clade A, member 1; Serpin A1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)