Home / Serpin A3/alpha 1-Antichymotrypsin / Human Serpin A3/ alpha 1-Antichymotrypsin Antibody

Human Serpin A3/ alpha 1-Antichymotrypsin Antibody

Catalog #: AF1295
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1295
AF1295-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin A3/ alpha 1-Antichymotrypsin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin A3/ alpha 1‑Antichymotrypsin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A3/ alpha 1‑Antichymotrypsin
Asn26-Ala423
Accession # P01011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Serpin A3/ alpha 1-Antichymotrypsin (Catalog # 1295-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Serpin A3/
alpha 1-Antichymotrypsin (Catalog # 1295-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A3/alpha 1-Antichymotrypsin

Serpin A3 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma (2). It is known to inhibit several serine proteases including chymotrypsin, cathepsin G, chymase, kallikrein 3/prostate specific antigen, and unidentified ectoenzymes that process pro-macrophage stimulating protein (1‑5). Serpin A3 is a major constituent of the plaques associated with Alzheimer’s disease and an inhibitor of amyloid beta peptide degradation (1‑6). Deficiency in Serpin A3 activity due to a point mutation (Leu55Pro) is associated with chronic obstructive pulmonary disease (7). Human Serpin A3 is synthesized as a 423 amino acid precursor (8, 9). The mature protein is secreted and has two forms that differ in their N‑termini (10). One of the forms starting at Asn26 was expressed and purified.

References
  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Kalsheker, N. et al. (2002) Biochem. Soc. Trans. 30:93.
  3. Duranto, J. et al. (1998) Biochemistry 37:11239.
  4. Hsieh, M.-C. and B.S. Cooperman (2002) Biochemistry 41:2990.
  5. Skeel, A. and E.J. Leonard (2001) J. Biol. Chem. 276:21932.
  6. Abraham, C.R. et al. (2000) Ann. N. Y. Acad. Sci. 920:245.
  7. Gooptu, B. et al. (2000) Proc. Natl. Acad. Sci. USA 97:67.
  8. Chandra, T. et al. (1983) Biochemistry 22:5055.
  9. Morii, M. and J. Travis (1983) J. Biol. Chem. 258:12749.
  10. Lindmark, B. et al. (1989) Biochim. Biophys. Acta 997:90.
Entrez Gene IDs
12 (Human)
Alternate Names
AACTMGC88254; ACTGIG25; alpha 1-Antichymotrypsin; alpha-1-antichymotrypsin; antitrypsin), member 3; Cell growth-inhibiting gene 24/25 protein; growth-inhibiting protein 24; growth-inhibiting protein 25; member 3; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; serine (or cysteine) proteinase inhibitor, clade A, member 3; Serpin A3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

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Citations for Human Serpin A3/ alpha 1-Antichymotrypsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. S-glutathionylated serine proteinase inhibitors as plasma biomarkers in assessing response to redox-modulating drugs.
    Authors: Grek C, Townsend D, Uys J, Manevich Y, Coker W, Pazoles C, Tew K
    Cancer Res, 2012-03-08;72(9):2383-93.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  2. Increased Serpina3n release into circulation during glucocorticoid-mediated muscle atrophy
    Authors: Marine Gueugneau, Donatienne d'Hose, Caroline Barbé, Marie de Barsy, Pascale Lause, Dominique Maiter et al.
    Journal of Cachexia, Sarcopenia and Muscle

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