Human Serpin A8/Angiotensinogen Biotinylated Antibody

Catalog #: BAF3156
1 Citation Datasheet / COA / SDS

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BAF3156

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin A8/Angiotensinogen Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serpin A8 in Western blots. In this format, less than 1% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A8
Asp34-Ala485
Accession # ABM85122

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Serpin A8/Angiotensinogen

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A8/Angiotensinogen

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II. Ang II induces vascoconstriction and aldosterone release. Mature human angiotensinogen is 452 aa in length (aa 34-485). It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41). Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.

Entrez Gene IDs

183 (Human); 11606 (Mouse); 24179 (Rat)

Alternate Names

AGT; alpha-1 antiproteinase, antitrypsin; Angiotensin II; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Angiotensinogen; ANHU; Aogen; FLJ92595; FLJ97926; PAT; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; SERPINA8angiotensin I

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Serpin A8/Angiotensinogen Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1