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Human Serpin B5/Maspin Antibody

Catalog #: AF2218
1 Citation Datasheet / COA / SDS
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AF2218
AF2218-SP
Detection of Human Serpin B5/Maspin by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin B5/Maspin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin B5/Maspin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin C1, rhSerpin F2, rhSerpin I1, rhSerpin I2, rhPEDF, and recombinant mouse Serpin D1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Serpin B5/Maspin
Met1-Pro375
Accession # P36952
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Knockout Validated
Serpin B5/Maspin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Serpin B5/Maspin knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Serpin B5/Maspin antibody by Western Blot. View Larger

Detection of Human Serpin B5/Maspin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated Western Blot Shows Human Serpin B5/Maspin Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Serpin B5/Maspin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Serpin B5/Maspin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B5/Maspin

Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. The amino acid sequence of human Serpin B5 is 99%, 92%, 89%, 88% and 64% identical to that of chimpanzee, dog, mouse, rat and chicken.

Entrez Gene IDs
5268 (Human)
Alternate Names
Maspin; Peptidase inhibitor 5; PI5; PI-5; PI5protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin peptidase inhibitor, clade B (ovalbumin), member 5

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Citation for Human Serpin B5/Maspin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration.
    Authors: Bass R, Wagstaff L, Ravenhill L, Ellis V
    J. Biol. Chem., 2009-07-28;284(40):27712-20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot

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