Human SHBG Antibody

Catalog #: MAB26562 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB26562-100
MAB26562-SP

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Human SHBG Antibody Summary

Species Reactivity

Human

Specificity

Detects human SHBG in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 336729

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SHBG
Leu30-His402
Accession # P04278

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26561).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SHBG DuoSet ELISA Kit (Catalog # DY2656) for convenient development of a sandwich ELISA or the Human SHBG Quantikine ELISA Kit (Catalog # DSHBG0B) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human SHBG ELISA Standard Curve. Recombinant human SHBG protein was serially diluted 2-fold and captured by Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHBG

Human Sex Hormone Binding Globulin (SHBG), also known as ABP or androgen binding protein, is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 aa long laminin a chain G-like domains. Each SHBG monomer is a 47‑53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.

Long Name

Sex Hormone-binding Globulin

Entrez Gene IDs

6462 (Human)

Alternate Names

ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin