Human Siglec-15 Antibody

Catalog #: MAB9227 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9227-100
MAB9227-SP

Detection of Siglec-15 in HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Siglec-15 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1026113

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Siglec-15
Phe20-Thr263
Accession # Q6ZMC9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec-15 in HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-15 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human Siglec-15 monoclonal antibody (Catalog # MAB9227) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

References

Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
Angata, T. et al. (2007) Glycobiology 17:838.
Takamiya, R. et al. (2013) Glycobiology 23:178.
Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Alternate Names

CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15