Human Siglec-16 Antibody

Catalog # Availability Size / Price Qty
MAB68192-100
MAB68192-SP
Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry
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Human Siglec-16 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec-16 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 706030
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Siglec-16 peptide
Accession # A6NMB1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 human embryonic kidney cell line transfected with human Siglec-16 and eGFP
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
CHO cells transfected with human Siglec-16 or irrelevant transfectant

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human Siglec-16 and eGFP was stained with (A) Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68192) or (B) Mouse IgG1 Isotype Control Antibody (MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).Staining was done using our Staining Membrane-associated Proteins protocol.

Intracellular Staining by Flow Cytometry View Larger

Detection of Siglec‑16 in CHO cells transfected with Human Siglec-16 or irrelevant Transfectant cells by Flow Cytometry. CHO cells transfected with human Siglec-16 (filled histogram) or irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68192), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-16

Siglec-16 (Sialic acid-binding Ig-like lectin 16) is a 58-60 kDa member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an alpha 2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM) Lys that interacts with DAP12. Mature human Siglec-16 is a 465 amino acid (aa) type I TM glycoprotein. It contains a 418 aa extracellular region (aa 17-434) that shows one V-type (aa 19-122) plus three C2-type (aa 147-424) Ig‑like domains, and a short 26 aa cytoplasmic tail. Notably, Siglec-16 exists as a pseudogene in approximately 50% of surveyed population. There is no Siglec-16 counterpart in either rodent or distant primate such as Rhesus monkey. The extracellular domain (ECD) of human Siglec-16 shares 94% aa identity with the ECD of human Siglec-11.

Long Name
Sialic Acid Binding Ig-like Lectin 16
Entrez Gene IDs
400709 (Human)
Alternate Names
Siglec16; Siglec-16; SIGLECP16; Siglec-P16

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