Human Siglec-16 Antibody

Catalog #: MAB68191 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB68191-100
MAB68191-SP

Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry

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All Siglec-16 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-16 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Siglec-16 in direct ELISAs. In flow cytometry, detects human Siglec-16, but not human Siglec-11, in transfected HEK293 cells.

Source

Monoclonal Mouse IgG_2B Clone # 706045

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Siglec-16 peptide
Accession # A6NMB1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP and CHO cells transfected with human Siglec-16 or irrelevant transfectant cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

CHO cells transfected with human Siglec-16 or irrelevant transfectant

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human Siglec-16 or (B) human Siglec-11, and eGFP was stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68191) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).View our protocol for Staining Membrane-associated Proteins.

Intracellular Staining by Flow Cytometry

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Detection of Siglec‑16 in CHO cells transfected with human Siglec-16 or irrelevant transfectant cells by Flow Cytometry. CHO cells transfected with human Siglec-16 (filled histogram) or irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68191), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-16

Siglec-16 (Sialic acid-binding Ig-like lectin 16) is a 58-60 kDa member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an alpha 2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM) Lys that interacts with DAP12. Mature human Siglec-16 is a 465 amino acid (aa) type I TM glycoprotein. It contains a 418 aa extracellular region (aa 17-434) that shows one V-type (aa 19-122) plus three C2-type (aa 147-424) Ig‑like domains, and a short 26 aa cytoplasmic tail. Notably, Siglec-16 exists as a pseudogene in approximately 50% of surveyed population. There is no Siglec-16 counterpart in either rodent or distant primate such as Rhesus monkey. The extracellular domain (ECD) of human Siglec-16 shares 94% aa identity with the ECD of human Siglec-11.

Long Name

Sialic Acid Binding Ig-like Lectin 16

Entrez Gene IDs

400709 (Human)

Alternate Names

Siglec16; Siglec-16; SIGLECP16; Siglec-P16