Human Sigma-1 R Antibody

Catalog #: MAB1076 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1076-100
MAB1076-SP

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Human Sigma-1 R Antibody Summary

Species Reactivity

Human

Specificity

Detects human Sigma-1 R in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1319A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing human Sigma-1 R
Accession # Q99720

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Sigma-1 R in HeLa Human Cell Line. Sigma-1 R was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Sigma-1 R Monoclonal Antibody (Catalog # MAB1076) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sigma-1 R

Sigma non-opioid intracellular receptor 1 (SIGMAR1, OPRS1 or Sigma1 receptor) is a 223 aminoacids (aa), 25KDa chaperone protein at the endoplasmic reticulum (ER) that modulates calcium signaling through the IP3 receptor. Sigmar1 functions as a lipid transporter and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. In Human, at least 4 other isoforms have been found, ranging from 12-22 KDa.

Long Name

Sigma Non-opioid Intracellular Receptor 1

Entrez Gene IDs

10280 (Human); 18391 (Mouse); 29336 (Rat)

Alternate Names

Aging-associated gene 8 protein; ALS16; FLJ25585; hSigmaR1; OPRS1; SIG-1R; sigma 1; Sigma 1-type opioid receptor; sigma non-opioid intracellular receptor 1; Sigma-1 R; sigma1 receptor; Sigma1R; Sigma-1R; sigma1-receptor; SIGMAR1; SR31747 binding protein 1; SR31747-binding protein; SRBP; SR-BP; SR-BP1; SRBPMGC3851; type I sigma receptor